Using multiplex nucleotide barcoding married to proprietary molecular biology and bioinformatics, Atreca’s proprietary Immune Repertoire Capture® (IRC™) and DeepSeq™ technologies provide unbiased and virtually error-free antibody and T cell receptor (TCR) sequences from immune responses in humans and other species. The exceptional data quality delivered by both IRC™ and DeepSeq™ technologies enables accurate quantitation of immune responses, including accurate measurement of clonal frequencies, the tracking of sequence and clonal evolution over time, and identification of convergent evolution across multiple individuals.
IRC™ generates immune repertoire information from single B or T cells of key subpopulations with key advantages:
Any sequence delivered by Atreca’s IRC™ technology is ready for immediate gene synthesis, expression, and testing, without the need for re-sequencing or re-cloning. The resulting functional proteins can be used directly in a wide variety of downstream applications, including identifying the targets of immune responses.
For B cells, IRC™ defines precise, natively paired heavy and light chain sequences for the antibodies encoded by a wide variety of selected, single B cells. The sequences generated from each B cell completely specify an exact antibody, from the signal sequence through to the constant region, enabling straightforward generation of recombinant antibody that exactly reproduces the amino acid sequence present in vivo.
For T cells, IRC™ delivers natively paired chains and error-corrected sequences from single T cells, just as for B cells, but with the addition of single-cell phenotype data. Given the diverse biological functions of T cells, it is critically important to know not only the TCR sequence but also the function of the T cell which expresses that TCR. IRC™ delivers a phenotypic profile for each cell by interrogating classical protein markers.
Atreca’s DeepSeq™ technology delivers information about gene expression in B or T cells, again with error-corrected sequences and unbiased output.
Applied to human disease, Immune Repertoire Capture® and DeepSeq™ technologies together are an engine for the discovery and development of therapeutics, vaccines, and diagnostics in cancer, infectious disease, and autoimmune disease.
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